Many authors came to the same conclusion a decade ago aumaitre et al. The effects of bt and near isogenic lines of rice were studied in two laboratory experiments. The four modern upland cotton genotypescultivars were selected in 1995, and were. We provide evidence for structural and detoxificationrelated mechanisms underlying. However, little is known regarding the rpsinduced molecular responses to p. Faust, 2002, suggesting that little contradictory data has emerged over the past 10 yr, despite the increased global prevalence of ge feed. A program to develop near isogenic lines nils of rice with single resistance genes was begun at the international rice research institute ir ri in the philippines. Each substitution line is nearly isogenic to the common parent tm1 for 25 chromosome pairs. The fact of similar genetic background and differences at the locus of a target gene between nils would facilitate to analyze the response to pathogen in resistance and susceptible line as well as to compare the difference between. Genome sequencing of spinach near isogenic line 1 nil1. Zeigler2, surapong sarkarung2,2, itsuro takamurel and toshiro kinoshita 1 plant breeding institute, faculty of agriculture. Identification of molecular markers linked to leaf rust resistance genes in wheat and their detection in the local nearisogenic line. Use of nearisogenic lines derived by backcrossing or selfing to map. We thus sequenced, analyzed, and compared the transcriptomes of 10 near isogenic lines nils, each with a unique rps geneallele, and the.
Transcriptome analysis of wheat and barley nearisogenic. Each of the 89 nils, the control line, tx303, and b73 was test crossed to the inbred line mo17 for evaluation. Reciprocal qtlspecific near isogenic lines qtlnils have been developed that combine two qtl, rphq 2 and rphq 3, the largest effects. Black shank resistance and leaf surface chemistry of flue. Generation of multiple near isogenic lines and rnaseq analysis we generated multiple nils segregating for the 4a1 qtl following a method based on hifs analysis 8. M siddique1 5 guijun yan111 6 1uwa school of agriculture and environment and the uwa institute of agriculture, 7 the university of western australia, perth wa 6009. These intermediate barley near isogenic ibison lines represent disease resistance qtl combined in one, two, and threeway combinations in a susceptible background. Some features of this site may not work without it. Review article incident duration modeling using flexible. Phytophthora root and stem rot prr of soybean, caused by phytophthora sojae, is controlled by rps genes. Identification of molecular markers linked to leaf rust. Pdf nearisogenic lines nils constitute valuable tools in genetic investigations and plant breeding programs. To understand the physiological processes responsible for elevated cd accumulation in shoots and grain, cd uptake and translocation were studied in seedlings of a pair of near isogenic durum wheat lines, high.
Following the previous identification of a major qtl for tgw within the interval of 15. Here we introduce the development of a genomewide coverage near isogenic line nil population of arabidopsis thaliana, by introgressing genomic regions from the cape verde islands cvi accession into the landsberg erecta ler genetic background. To determine the role of glutopeak parameters in assessing wheat quality, near isogenic lines nils of three wheat varieties with different gluten strengths were used in this study. Qtl mapping with nearisogenic lines in maize springerlink. Comparative analysis of root transcriptome profiles of two pairs of droughttolerant and susceptible rice near isogenic lines under different drought stress ali moumeni, 1, 2 kouji satoh, 1 hiroaki kondoh, 1 takayuki asano, 1 aeni hosaka, 1 ramiah venuprasad, 3, 4 rachid serraj, 3, 5 arvind kumar, 3 hei leung, 3 and shoshi kikuchi 1. The purpose of developing near isogenic lines is to establish a set of international differentials with monogenic base of the resistance to bacterial leaf blight pathogen and to supply reliable materials to breeding programs for resistant varieties. To verify previously reported associations we evaluated the effects of nine don qtl using near isogenic lines nils for each qtl region. Although nearisogenic lines nils can standardize genetic backgrounds among individuals, it has never. Families of contrasting homozygous haplotypes for each region were derived from lines in the original am populations that were heterozygous for don qtl. Chlorophyll retention in nearisogenic lines of soybeans. Small differences between the ssr banding patterns of a dwarf seedling compared to the normalphenotype seedling from the same seed, may indicate the loss or distortion of part of a chromosome near isogenic lines or a whole chromosome monosomic aneuploidy. This work describes the development of maize recombinant near isogenic lines nils for the mendelization of six heterotic qtl previously identified based on a maize zea mays l.
Near isogenic lines nils are derived via repeated backcrossing of genetically distinct parental lines, most commonly with the aim of transferring a single chromosomal region of the donor parent. Nearisogenic lines nils, pairs of lines that are identical except for a single genomic segment, are ideal for this purpose szalma et al. Isogenic definition is characterized by essentially identical genes. These two parents were used to produce a set of near isogenic lines nils with small regions of introgression into both backgrounds. Dk 440 bty e transformation event mon810 or with its near isogenic line dk 440 on chernozem soil.
Objectives identified sources of quantitative or partial resistance to black shank developed near isogenic lines evaluated two quantitative trait loci qtls conferring black shank resistance evaluated leaf. Estimatingthegeneticarchitectureoffruitquality traits in. Construction and evaluation of nearisogenic lines for. Near isogenic line nil pairs carrying the resistant and susceptible allele for a wheat qtl and three barley qtl were used to examine don concentration, fungal biomass and transcript accumulation during f. Application of a library of near isogenic lines to understand context dependent expression of qtl for grain. The complex quantitative nature of droughtrelated traits is a major constraint to breed tolerant wheat varieties. Nearisogenic lines can be derived through repeated backcrossing to a recurrent parent. Transcriptome analysis of brassica rapa nearisogenic. Comparative analysis of root transcriptome profiles of two. Nearisogenic lines nils are a valuable resource for detecting linkages between qualitative trait loci and molecular markers. Chlorophyll retention in nearisogenic lines of soybeans welcome to the ideals repository. This study precisely mapped and validated a major quantitative trait locus qtl on chromosome 4al for thousandgrain weight in wheat using multiple near isogenic lines. The four modern upland cotton genotypescultivars were selected in.
Evaluation of low alkaloid tobacco lines for chlorophyll. Acknowledgement the authors wish to express sincere thanks to. Evaluation of low alkaloid tobacco lines for chlorophyll levels, flowering dates and relative ripening rates summers1, c moore1, j. The plant lines used were nils 442 winter wheat and 443 spring wheat, which should differ only in the vrn1fr1 region of chromosome 5a. Nearisogenic wheat lines in which one or more of these loci were absent or deleted were used. The relationships between development and low temperature. Transcriptomic analysis of wheat nearisogenic lines. A microarray analysis of rice nearisogenic lines that. Registration of four nearisogenic soybean lines of g0032 for resistance to asian soybean rust zachary r. Molecular response to the pathogen phytophthora sojae. Ideogram showing the location of fruit quality trait loci, located by the means of nearisogenic lines on the melon genetic map. Cadmium cd concentrations in durum wheat triticum turgidum l. Gm variety using transcriptomics, proteomics and metabolomics.
A novel arraybased genotyping platform was used to score genotypes of over 7,000 loci in 100 nils with b73. Near isogenic lines nils were applied for the first time to microarray analysis of 8,987 randomly selected expressed sequence tags ests aiming at screening rice genes associated with seed callus proliferation. Journal of crop science and biotechnology 263 markerassisted foreground and background selection of near isogenic lines for bacterial leaf pustule resistant gene in soybean kil hyun kim1, moon young kim1, kyujung van1, jungkyung moon2, dong hyun kim1, sukha lee1, 3 11department of plant science and research institute for agriculture and life sciences, seoul national university, seoul, 151. Identification of melon fruit quality quantitative trait loci using. Quantitative trait locus mapping using sets of nearisogenic lines. Markerassisted foreground and background selection of. In this study, near isogenic lines nils for 17 drought resistancerelated qtl were constructed and phenotypic variations of these nils were investigated under drought and normal conditions. To evaluation of pathogenicity and race classification of xanthomonas oryzae pv. Registration of four nearisogenic soybean lines of g00. A retention zone a pollen capture crop surrounding the entire test. Wl711 developed at the school of biotechnology were used along with the donor and the recurrent parents for identifying rapd markers linked to the two genes. Cadmium uptake and translocation in seedlings of near. Alteration of antioxidant enzyme gene expression during. Author summary gray leaf spot gls, a necrotrophic, foliar fungal disease of maize, contributes to maize yield losses worldwide.
Application of a library of near isogenic lines to understand context. We identified and characterized regions of the maize genome that confer resistance to gls and gained insight into the mechanisms associated with these quantitative trait loci qtl. Nil 442 vrn1vrn1fr1fr1 displays a winter wheat habit and nil 443 vrn1vrn1fr1fr1 displays a spring wheat growth habit 25. Development of nearisogenic lines in a parthenogenetically. Phenotypic and genotypic characterization of nearisogenic. Numerous quantitative trait loci qtl have been previously mapped using a recombinant inbred line population derived from these two genotypes. The efficient and inexpensive strategy adopted to generate sets of nils. Review article incident duration modeling using flexible parametric hazardbased models ruiminliandpanshang institute of transportation engineering, department of civil engineering, tsinghua university, heshanheng building, tsinghua. A qtl associated with reduced deoxynivalenol don accumulation was identified on chromosome 3h bin 6. The control line was derived from the same cross and backcrossing procedures as described above, but was homozygous for the b73 allele at each of the 19 rflp loci. Callus proliferation of the variety koshihikari is. Growth, reproduction, and superoxide dismutase activity were unaffected both in the petridish and soil microcosm experiment. In the first described experiment, we measured four components of disease resistance on the ibison lines.
Use of nearisogenic wheat lines to determine the glutenin. Utilization of nearisogenic lines to identify genes. If the inline pdf is not rendering correctly, you can download the pdf file here. Tortillas were prepared from each deletion line and the parent lines. Tarc repot selection of recurrent parents to develop near. The elimination of certain hmwgs alleles alter distinct but critical aspects of tortilla quality such as diameter, shelf stability, and overall quality.
The development and application of nearisogenic lines for. Use of nearisogenic lines to precisely map and validate a. In addition, a pair of nearisogenic lines nilsa clubrootresistant cr bjn32 line and a clubrootsusceptible bjn32 linewere developed for b. Joint analysis of nearisogenic and recombinant inbred. Five f 7 rils from the mapping population were chosen as founders of hifs on the basis of being heterozygote for the 4a1 qtl region fig. The glutopeak is a new instrument used to measure the rheological properties of wheat flour dough. Outline downy mildews from r gene perspective resources and mapping rpf1 locus current status future directions. Cottonseed protein, oil, and mineral nutrition in near. Such a set of nils would offer several advantages to researchers studying rice blast 1992 the american phytopathological society 746 phytopathology backcrossing. By comparing the profiles of the two transgenic lines grown in the same location over. Cucumis melo, introgression line, qtl, g e interaction, heritability abstract. The power to detect qtl using nearisogenic line nil comparisons versus recombinant inbred ri populations was assessed. The expression of cbf genes in hexaploid wheat was investigated by comparing the response to cold acclimation of four near isogenic lines previously generated from two cultivars, spring manitou a. Differential gene expression in nearly isogenic lines with.
Research open access transcriptomic analysis of wheat nearisogenic lines identifies pm19a1 and a2 as candidates for a major dormancy qtl jose m. Multiple nearisogenic lines targeting a qtl hotspot of. We characterized a set of isogenic genetic stocks representing single and pairwise substitutions of spring alleles at the. Pdf the development of near isogenic lines nils through repeated backcrossing of genetically distinct parental lines is rather. The barleypuccinia hordei barley leaf rust pathosystem is a model for investigating partial disease resistance in crop plants and genetic mapping of phenotypic resistance has identified several quantitative trait loci qtl for partial resistance. Construction and evaluation of near isogenic lines for major qtls of basal root thickness and grainweight in lowland and upland rice volume 4 issue 3 liu lifeng, zhang hongliang, mu ping, qu yanying, li zichao. The near isogenic lines for virescent yellow and wildtype green leaf sets were developed by a backcross breeding method using four modern upland cotton varieties line development was detailed by others 35. Reducing sugars for nc 95 and three near isogenic lines at each harvest trt hvst1 % hvst2 % hvst3 % hvst4 % nc 95 16. Although heterosis is widely exploited in agriculture, a clear understanding of its genetic bases is still elusive. Peiffer, gregory allan, utilization of nearisogenic lines to identify genes underlying ironefficiency qtl 2011. Molecular response to the pathogen phytophthora sojae among ten soybean near isogenic lines revealed by comparative transcriptomics by feng lin, meixia zhao, douglas d baumann, jieqing ping, lianjun sun, yunfeng liu, biao zhang, zongxiang tang, elisa hughes, rebecca w. All of isolates were inoculated to assess the differential characteristics of 26 near isogenic rice lines containing a single resistance gene or two to.
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